Recorded Webinars

Excellence in Research webinar series

Your trusted resource for cell lines, microbial strains, biological reagents, and innovative solutions invites you to view one of the exciting webinars in our Excellence in Research series. Each webinar featured a highly experienced ATCC Scientist who demonstrated how to utilize ATCC products and services to advance your research. The webinars were broadcasted live, ran 30 to 45 minutes, and were followed by a 10 minute question and answer period.

2015 Excellence in Research Series

Liz KerriganMicrobiology Quality Control as Described in the Compendia

Scott Sutton, Ph.D., Principal, Microbiology Network, Inc.
Liz Kerrigan, Director, Product Development, Sales and Marketing, ATCC
Recorded March 19, 2015

The quality control microbiology lab serves a valuable function in the pharmaceutical manufacturing environment in terms of providing information on the biological quality of the products being produced.  The major tests are familiar to us all – Microbial Limits, Sterility Tests, and Antimicrobial Effectiveness Tests.  All of these tests require strict attention to detail for reproducible results, and the compendium also provides guidance on the quality control aspects of laboratory operations.  In the first half of this presentation, Dr. Sutton will provide a brief overview of both of these aspects of microbiological quality control with guidance for the listener on how to further research best practice as described in the compendium. In the second half, Mrs. Kerrigan will describe how ATCC is striving to meet the current needs of the quality control microbiology lab. Download Presentation (.pdf) View Recording

David ClawsonEnhancing Vector-borne Research with Biological and Molecular Standards

David Clawson, M.S., Lead Biologist, ATCC
Recorded March 5, 2015

Vector-borne diseases are a major public health concern, affecting billions of people worldwide. Due to the complexity of vector-borne pathogen transmission, these illnesses are among the most difficult infectious diseases to predict, prevent, and control. Moreover, many vector-borne pathogens can be challenging to culture, require high-containment facilities, or are on the commerce control list, making them difficult to study. To support the development of rapid diagnostic tools and innovative therapeutics, ATCC has synthetically derived nucleic acids that represent key target regions from a number of infectious microorganisms, including dengue virus, West Nile virus, and Eastern equine encephalitis virus, among others. These standards are quantitated, stable, can be handled in BSL-1 conditions, and don’t require permits for international shipping. In this webinar, we will discuss emerging vector-borne microbial pathogens, molecular and biological products from ATCC that support vector-borne research, and will demonstrate the use of ATCC synthetic molecular standards microbial detection and quantification. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.

James ClintonDiscovering ATCC Hematopoietic Progenitor Cells – Model Systems to Study the Immune and Cardiovascular Systems

James Clinton, Ph.D., Scientist, ATCC
Recorded February 19, 2015

The cellular components of blood originate in bone medullary cavities. In the process of becoming fully functional, hematopoietic cells undergo a program of differentiation which begins in the marrow and may be completed in the peripheral tissues and organs such as blood, lymph, thymus, and spleen. The result is a diversity of cell types, each of which displays specific transport, hemostatic, and immune functions. Hematopoietic research tools have high value for investigating the pathogenesis of anemia and autoimmune diseases, and are useful controls in liquid tumor studies. In this webinar, ATCC scientists will discuss recent developments in developing models of hematopoiesis using immunological cells such as CD34+ bone marrow cells, CD14+ peripheral blood monocytes, and primary peripheral blood mononuclear cells. Download Presentation (.pdf) View Recording.

Tracey NoblettMatthew WhettonAccuracy Matters – Proficiency Testing Programs Offer Dependable Materials for Assessments with Trackable Online Results

Tracey Noblett, M.B.A., Head of Microbiology, Proficiency Testing, LGC and
Matthew Whetton, Ph.D., Head of Chemistry, Proficiency Testing, LGC
Recorded February 5, 2015

LGC is a leading life sciences measurement and testing business that offers a comprehensive range of products and services, including proficiency testing programs from LGC’s custom-built facility in the north of England. This presentation will discuss the general requirements and purpose of proficiency testing with reference to ISO/IEC 17043:2010. There will also be a brief overview of the different programs and samples offered by LGC, and available from ATCC in the U.S., including program logistics, sample preparation and quality control, and the statistical assessment and reporting of results. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording.

2014 Excellence in Research Series

John Pulliam3D Tissue Modeling

John Pulliam, Ph.D., Field Application Scientist, ATCC
Recorded November 13, 2014 

The emergence of 3D tissue modeling raises new possibilities for the study of complex physiological processes in vitro. Advances in cell isolation, media development, substrates, and growth surfaces are leading to protocols that provide more functionality than traditional 2D cell culture. These models may provide a more predictive analysis and result in a more streamlined process of drug discovery and development. In this webinar, we will discuss recent developments in 3D modeling using ATCC primary and hTERT immortalized cells in areas such as angiogenesis, wound healing, and respiratory studies. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording

Tigwa DavisPrecise Counting of Targeted Nucleic Acids has Never Been Easier

Francisco Bizouarn, Global Digital Applications Specialist, Digital Biology Center, Bio-Rad Laboratories
Recorded October 30, 2014 

Droplet Digital PCR (ddPCR) is an elegant technology that permits accurate and absolute quantification of target nucleic acid molecules. By subpartitioning a PCR reaction into thousands of nanoliter sized droplets, amplifying, and subsequently individually interrogating them, ddPCR allows for increased quantitative resolution, enhanced detection of difficult to amplify targets, better reproducibility, and simplified data analysis. This webinar will provide a technical overview of ddPCR from technical and workflow related perspectives as well as review key applications such as copy number variation (CNV) analysis, rare mutation detection (RMD) analysis, and standard free (stand-alone) absolute quantification of nucleic acids. Download Presentation (.pdf) View Recording

Tigwa DavisUsing LUHMES Cells as a Model System to Study Dopaminergic Neuron Cell Biology
Tigwa H. Davis, Ph.D., Senior Scientist, Neurobiology, ATCC
Recorded October 16, 2014 

Dopaminergic neurons play significant roles in motor, reward, and motivational behavior related circuits throughout the brain. To date, there are few continuous in vitro models available to laboratories in research, industry, and academia for studies related to basic dopaminergic cell biology or high throughput screening. Here, we propose the use of a human model system, LUHMES cells, to study dopaminergic neuron cell biology. During this webinar, we will highlight some of the advantages of using LUHMES cells, as well as examples of how they have been used in drug screening and to study the molecular mechanisms related to Parkinson’s Disease. Download Presentation (.pdf) View Recording

Fang TianDavid H. RandleATCC® Genetic Alteration Cell Panels: Effective Tools for High Throughput Screening Using Corning® Epic® Technology

Fang Tian, Ph.D., Lead Scientist, ATCC
David H. Randle, Ph.D., Manager, Applications Development, Corning Life Sciences
Recorded September 18, 2014 

Extensive genomic™ sequencing efforts in recent years have provided detailed profiles of the somatic gene mutations that occur in a wide range of human cancers. In order to facilitate basic and translational cancer research, ATCC has designed and validated a number of Genetic Alteration Cell Panels targeting the key molecular pathways identified in these studies. To demonstrate suitability of the panels for high throughput screening, the EGFR panel was selected for evaluation using Corning Epic Technology, a label-free platform that uses optical biosensors for high sensitivity biochemical and cell-based assays. In this webinar, we will discuss how the combination of Epic Technology and the EGFR Genetic Alteration Panel offers convenient tools to screen for ligands or biologics that directly target or affect EGFR receptor biology. Download Q&A Session (.pdf)   Download Presentation (.pdf) View Recording

Shamaila Ashraf ATCC® Influenza Research Materials
Shamaila Ashraf, M.Sc, M.Phil, Ph.D., Senior Scientist, ATCC
Recorded September 11, 2014

Influenza remains one of the most significant infectious diseases worldwide, causing acute respiratory tract illnesses and accounting for 25% of infections that exacerbate chronic lung infections. To date, several epidemics and four major influenza pandemics have been recorded. Influenza viruses have caused an estimated 3 million cases of serious illness and around 500,000 deaths annually worldwide. Influenza infections are primarily and effectively controlled by vaccines that elicit protective immunity. Influenza viruses undergo rapid antigenic shift and drift that results in the emergence of new strains each year. Therefore, influenza vaccines need to be reformulated every year to match the circulating strains. In this webinar, we will provide an overview of the influenza virus and will explore the current treatment strategies for influenza infections. We will also highlight viral strains and associated materials offered by ATCC that can be used in influenza research or in the development and validation of novel preventative and therapeutic techniques. Download Q&A Session (.pdf)  Download Presentation (.pdf) View Recording

Yukari TokuyamaATCC® Human Pluripotent Stem Cells – Enabling Research through Standards
Yukari Tokuyama, Ph.D., Field Application Scientist, ATCC
Recorded August 21, 2014 

Induced pluripotent stem cells (iPSCs) provide a powerful tool to model human disease in relevant cell types. iPSCs may be generated from patients of any genetic background, and possess the capacity to differentiate into almost any desired terminal cell type. In this webinar, we will focus on ATCC's approach in generating and providing standardized, quality controlled, and highly characterized human iPSCs lines with its complete culture systems. We will also highlight the characterization of LRRK2 Parkinson’s patient-derived iPSC lines by whole-exome sequencing. Download Q&A Session (.pdf)  Download Presentation (.pdf) View Recording

Douglass R. Storts, Ph.D.Yvonne ReidSTR DNA Profiling – The Standard for Cell Line Authentication
Douglas R. Storts, Ph.D., Head of Research - Nucleic Acid Technologies, Promega Corporation and
Yvonne A. Reid, Ph.D., Manager/Scientist, Cell Biology Program, ATCC
Recorded June 5
, 2014 

Misidentification of human cell lines is a common problem that leads to irreproducible research in the Life Sciences.  Over the years, use of contaminated cell lines has increased due to poor techniques, inadequate authentication protocols, and sharing of false cell lines among researchers.   These concerns can be readily resolved by the implementation of the Standard STR protocol –authentication of human cell lines by STR profiling. In this webinar, we will discuss the recent advances in STR profiling technologies and will delve into further detail on how the Standard STR protocol is transforming scientific practices. Download Q&A Session (.pdf)  Download Presentation (.pdf) View Recording

Liz KerriganThe Importance of Standards in Molecular-Based Assays
Liz Kerrigan, Director, ATCC Standards
Recorded May 8, 2014 

Stimulated by the presence of emerging and re-emerging microbial threats, the need for molecular-based tests has significantly increased in the last few years. In turn, this has necessitated the use of standardized nucleic acids in clinical laboratories. In this webinar, we will discuss the importance of molecular standards in the establishment of sensitivity, linearity, and specificity during assay validation or implementation, quality assurance, quality control, and proficiency testing, and how the use of standards can contribute to improvements in assay reproducibility and reliability. Download Q&A Session (.pdf)  Download Presentation (.pdf) View Recording

Fang Tian ATCC Breast Cancer Research Resources
Fang Tian, Ph.D., Lead Scientist, Cell Biology Group Leader, ATCC
Recorded April 24, 2014 

Breast cancer is the leading cause of cancer-related mortality in women. New disease classifications, relevant signaling pathways, and genetic regulators of breast cancer have been identified over the past decade. To continue facilitating progress in basic research and drug discovery, ATCC provides breast cancer research resources. In this webinar, we will discuss triple negative breast cancer cell lines, breast cancer mouse models, and commonly used breast cancer in vitro models with in-depth genetic alteration and molecular profiles. We will also highlight cell lines that can be used to address recently identified genomic and clinical features of breast cancer subtypes. Download Q&A Session (.pdf)  Download Presentation (.pdf) View Recording

CK Zhang hTERT Immortalized Cell Lines – Unique Tools for Physiologically Relevant Research
Chengkang Zhang, Ph.D.
Recorded March 27, 2014 

Human telomerase (hTERT) immortalized cell lines combine the properties of primary cells and the long culture life of continuous cell lines. In this webinar, we will provide an overview of the ATCC hTERT Immortalized cell line collection, and will examine the use of immortalized renal epithelial cells, keratinocytes, and microvascular endothelial cell lines to demonstrate how hTERT immortalized cell lines can help investigators reach their goals. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording

Cara WilderDrug-Resistant Acinetobacter baumannii – A Growing Superbug Population
Cara Wilder, Ph.D., Technical Writer, ATCC
Recorded March 13, 2014 

In recent years, natural and societal selective pressures have led to the emergence of numerous antibiotic-resistant microbial strains, including multidrug- and pandrug-resistant strains of Acinetobacter baumannii. These strains have become a significant cause of nosocomial infection among immunologically compromised individuals, resulting in increased morbidity and mortality. This presentation will provide an overview of this escalating problem and will explore the current techniques used to identify drug-resistant A. baumannii strains, available therapies, and remaining concerns. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording

Authentication and Characterization of Animal Cell lines: Towards Best Practices in Cell Culture
Yvonne A. Reid, Ph.D., Manager/Scientist
Cell Biology Program,
Recorded February 27, 2014 

Over the years, numerous cell lines have been shown to be misidentified due in part to poor techniques, inadequate authentication protocols, and sharing of unauthenticated cell lines amongst researchers. Technological advances have given rise to improved capabilities. Cell line authentication and characterization now requires a comprehensive strategy that employs several complementary technologies for systematic testing for morphology, microbial contaminations, cellular cross-contamination, as well as functionality. An overview of the current technologies used to authenticate and characterize animal cell lines will be presented. Download Q&A Session (.pdf) Download Presentation (.pdf)  View Recording

John Pulliam ATCC Sophisticated Approaches to In Vitro Research
John Pulliam, Ph.D., Field Application Scientist, ATCC
Recorded February 6, 2014  

This webinar will provide an overview of the services that ATCC offers to the scientific community. We will also discuss the sophisticated approaches of utilizing Genetic Alteration Panels; cell lines which are grouped by tissue type or pathway mutation, hTERT immortalized cells, and the HEKPlus system for protein production. Download Q&A Session (.pdf) Download Presentation (.pdf) View Recording

2013 Excellence in Research series

Fang Tian ATCC Molecular Signature Panels – Powerful tools for the genomics age
Fang Tian, Ph.D., Lead Scientist, Cell Biology Group, ATCC
Recorded December 5, 2013 

Want to bring your research into the genomics age? In this webinar, we will describe the ATCC molecular signature panels, which were generated by combining together authenticated cell lines that contain critical gene copy number changes and site mutations identified by next-generation sequencing. These panels focus on key components of cell signaling pathways. The gene expression, protein expression and cellular localization of EGFR, AKT, PI3K, PTEN, p53, RAS, RAF, ERK, MYC and MET were studied and compared within a large number of cell lines with various genetic backgrounds. View Recording

Breathing rocks for fun and profit
Jeffrey A. Gralnick, Ph.D., Associate Professor of Microbiology, University of Minnesota
Recorded November 14, 2013 

Some bacteria have the ability to live by breathing certain oxide minerals. I will talk about one of the best understood model organisms for this process, Shewanella oneidensis, and how a detailed understanding of this microbe could lead to breakthroughs in the areas of energy and bioremediation. View Recording

ATCC Transfection Reagents - A better style of protein expression!
Recorded October 24, 2013

Transfections got you down? It’s true - some cells are harder to transfect than others, but ATCC is here to help. This webinar will describe how transfection reagents and culturing practices can affect the success of your experiments. In addition, we will demonstrate how our new transfection reagent, TransfeX, can help you transfect even the most notoriously difficult cells (i.e. stem cells, primary cells) and help you get your experiments going. View Recording

Cara WilderMycoplasma Detection – Protect your continuous cell cultures
Cara Wilder, Ph.D., Technical Writer, ATCC
Recorded October 10, 2013 

Are you sure your continuous cell cultures and media are free from contamination? Mycoplasma contamination affects roughly 15-35% of continuous cell cultures, resulting in deleterious effects including the induction of chromosomal abnormalities, the disruption of DNA and RNA synthesis, and the inhibition of both cell metabolism and growth rate. In this presentation, learn how to protect your cultures using the ATCC® Mycoplasma Detection Kit. Download Q&A Session (.pdf) View Recording

Primary Cell Solutions - Biologically relevant in vitro models
Recorded September 26, 2013 

Did you know, ATCC, your trusted source for tumor cell lines, also offers primary cells? In this webinar, we will describe how our broad offering of primary cells, optimized media systems and transfection reagents, can help you get your primary cell cultures up and running, so you can get your experiments going, and your research moving forward. View Recording

Cara WilderATCC Toolbox—Kick-start your semester!
Cara Wilder, Ph.D.,Technical Writer, ATCC
Recorded September 12, 2013 

The academic year has begun, but are you ready for it? Let ATCC help you set up your lab! In this presentation, we will discuss some of our products designed with teaching laboratories in mind. We will also provide helpful information and key features about our Culture Guides and the new Introduction to Microbiology Guide. Get your students on the fast track to success! Download Q&A Session (.pdf) View Recording

David S. Blehert, PhD Fungal disease, a 21st century challenge to bat conservation
David Blehert, Ph.D., Microbiologist, U.S. Geological Survey,
National Wildlife Health Center
Recorded June 13, 2013 

Since first discovered in 2007 near Albany, New York, bat white-nose syndrome has spread to 20 US states and five Canadian provinces, and the disease is estimated to have killed over five million insectivorous hibernating bats.  An outbreak of infectious disease among bats on the order of white-nose syndrome is without precedent.  This presentation will provide an overview of this novel emergent wildlife disease and explore the profound impacts it may have on bat conservation in the 21st century. View Recording

John PulliamStem Cell Solutions
John Pulliam, Ph.D., Marketing Application Scientist, ATCC
Recorded June 6, 2013 

ATCC is your trusted source for induced pluripotent stem cell (iPSC) technology!  This webinar will provide background on human iPSC and ATCC products and introduce the advantages of our new human iPSC products.  Finally, we will demonstrate helpful tips and solutions for using the ATCC feeder-free human iPSC culture system for the thawing, passaging and cryopreservation. View Recording

Introducing your new prokaryotic species
Recorded May 16, 2013  

For over 85 years, ATCC has been helping scientists store and distribute their prokaryotic strains. In that time we have developed considerable expertise in maintaining the strains deposited with us, but it is up to the depositor to name their species and ensure that the name is recognized by the research community. In this talk, we will go over the deposit and name validation process, providing tips and pointing out potential bumps in the road. You’ll also get a list of essential publications and useful web sites to help you make sure your new species is successfully introduced to the world. Resources for Nomenclature and Taxonomy (.pdf) Download Q&A session (.pdf)  View Recording

Carbapenem-Resistant Enterobacteriaceae (CRE) – A Growing Superbug Population
Recorded April 18, 2013 

In recent years, natural and societal selective pressures have led to the emergence of numerous antibiotic-resistant microbial strains, including carbapenem-resistant Enterobacteriaceae (CRE) expressing Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-β-lactamase (NDM-1).   Infection with these strains has become a leading cause of nosocomial infections among immune-compromised individuals, resulting in increased morbidity and mortality. This presentation will provide an overview of this escalating problem and will explore the current techniques used to identify CRE strains, available therapies, and remaining concerns. View Recording


2012 Excellence in Research series

Non-O157 Shiga Toxin-Producing Escherichia coli
Recorded November 15, 2012 

The global community has been experiencing food-associated outbreaks of non-O157 Shiga toxin-producing Escherichia coli (STEC) for nearly two decades. As a result, six non-O157 STEC strains were classified as adulterants by the Food Safety and Inspection Service branch of the U.S. Department of Agriculture in June 2012, requiring routine verification testing of raw beef manufacturing trimmings for serogroups O26, O45, O103, O111, O121, and O145. ATCC has recently accessioned representative strains (both toxigenic and non-toxigenic) for each serogroup from the CDC and now has them available for use as controls for diagnostics and process validation. This webinar will discuss the genetic and phenotypic methods used to characterize these strains. Download Q&A session (.pdf) View Recording

Updates to the ATCC Material Transfer and Deposit Agreement: How ATCC Helps You in Research
Recorded October 18, 2012 

Earlier this year, ATCC revised its Material Transfer Agreement, to enable researchers and external research sites to transfer key biological reagents derived from ATCC material, such as transfected cell lines and genomic DNA, to collaborators without seeking permission from ATCC or paying administrative transfer fees. Additionally, the ATCC Deposit Agreement has been updated to allow those depositing with ATCC to share in the financial success of a deposit by receiving a royalty stream, once a certain sales threshold has been reached. In this webinar, we will provide more details about these updated agreements, and address how these changes affect the research community. Download Q&A session (.pdf) View Recording

Assessing Novel Storage Techniques and Their Ability to Stabilize Nucleic Acids
Kurt Langenbach, PhD, Manager, Research Scientist, Cores, ATCC and
Maria Mayda, Scientist, Assay Development, ATCC
Recorded October 4, 2012 

Novel ambient temperature stable storage approaches offer the potential advantage that various materials, such as nucleic acids, could be subjected to a broad range of hostile temperatures without diminishing and perhaps even improving their performance in downstream applications. In this webinar, we will discuss some of the results generated through our technology assessment program that have focused on evaluating innovative methods for the collection, processing, preservation, and characterization of bio-specimens, including nucleic acids, at ambient temperature. Insights gathered from these studies will allow us to move beyond traditional biological sample storage concepts, enhance the quality and reproducibility of results, improve sample handling, and minimize the impact of deleterious events in cold chain management. Download Q&A session (.pdf) View Recording

Biological Resources of the ATCC Protistology Collection
Robert Molestina, PhD, Senior Scientist, ATCC
Recorded September 6, 2012 

Protists are eukaryotic microorganisms commonly studied due to their ecological relevance in aquatic food chains and their impact on human health. Protistology research and comparative studies are dependent on the accessibility of authenticated biological standards. To meet this need, the Protistology Collection at the ATCC houses the largest and most diverse repository of protist cultures in the world. The primary role of the collection has been the acquisition, authentication, preservation, and distribution of reference strains. Characterization of deposited cultures includes a variety of tests such as viability, purity, phenotypic properties, and genotypic analysis. Additionally, over the last decade, resources within the collection have expanded to include genomic DNA and custom services. This presentation will provide an overview of biological resources available in the ATCC Protistology Collection, a discussion on the methods of characterization, benefits of depositing, current research projects, and future perspectives. View Recording

Tumor Cell Panels in Cancer Research and Drug Discovery
Fang Tian, Senior Scientist, Cell Biology, ATCC
Recorded August 23, 2012 

Human cancer cell lines have been used as essential tools in both high-throughput drug screening and detailed molecular mechanism studies. This Webinar will focus on genetic characterization of human cancer cell lines currently used in laboratories. Additionally, it will introduce how to choose the best cell lines and cell line panels for biological understanding of top genetic alterations across tumor types, and for testing small molecules or biologics for cancer drug development. View Recording

Good Cell Culture Practices

Misidentification/cross-contamination, long-term subculturing and passage number, poor culture conditions and microbial contamination (mycoplasma). View Recording

Corning Logo - Free online cell culture and assay training webinars.

You are invited to a series of free web-based technical seminars on cell culture. Co-sponsored by ATCC and The Society for In Vitro Biology (SIVB), the webinars are designed to provide novel tips, best practices and proven techniques to help with cell culture research needs.