Rahnella aquatilis Izard et al.

1. Open vial according to enclosed instructions.
2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours.

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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