Treponema vincentii (ex Brumpt) Smibert

35580 ^™

Product category: Bacteria
Strain designation: LA
Type strain: No
Isolation source: Patient with periodontitis
Product format: Frozen

Documentation

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Preceptrol: No

Characteristics

Comments: Immune response

Handling information

Medium: ATCC Medium 1494: Modified NOS medium
Temperature: 37°C
Atmosphere: Anaerobic
Handling procedure: 1.        Under anaerobic conditions thaw the vial at room temperature.
2.        Under anaerobic conditions, transfer the entire contents of the vial to a broth tube of ATCC^® #1494 broth.
3.        Additional tubes of broth may be inoculated with 0.5 ml each of the suspension. You may also use 0.5 ml to inoculate a #1494 slant. Inoculate two Tryptic Soy Agar plates with 5% sheep blood (ATCC^® Medium #260). Incubate one blood plate anaerobically to check for colony formation and incubate the second plate aerobically to check for purity.
4.        Incubate tubes and plate under anaerobic conditions at 37°C. Incubate the second blood plate aerobically.
5.        After 2-3 days, growth should be evident by slight turbidity in the broth. To observe growth, examine a wet mount of the broth under phase microscopy. Cells do not Gram stain readily. This organism does not readily grow on agar. No growth should occur on the blood agar plate incubated aerobically.
Handling notes: The cells are long, filamentous, spiral-shaped and motile Gram negative rods.
          Additional information on this culture is available on the ATCC^® web site at www.atcc.org.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
·   Use of an anaerobic gas chamber, or
·   Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
·   Loose screw caps on test tubes in anaerobic chamber,
·   Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
·   Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

History

Deposited as: Treponema vincentii (ex Brumpt) Smibert
Depositors: RK Nauman
Chain of custody: ATCC <-- RK Nauman <-- M.A. Listgarten <-- B.E. Laughon
Type of isolate: Human
Cross references: GenBank AF132815 Treponema vincentii FlaA-1 (flaA-1) gene, partial cds.

GenBank AF033309 Treponema vincentii 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Mangan DF, et al. In vitro lymphocyte blastogenic responses and titers of humoral antibodies from periodontitis patients to oral spirochete isolates. Infect. Immun. 37: 445-451, 1982. PubMed: 7118245

Fenno JC, et al. Conservation of msp, the gene encoding the major outer membrane protein of oral Treponema spp. J. Bacteriol. 179: 1082-1089, 1997. PubMed: 9023187

Wyss C, et al. Treponema amylovorum sp. nov., a Saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int. J. Syst. Bacteriol. 47: 842-845, 1997.

For product-related inquiries and issues, contact Technical Service:

Message Us

Send us a message

Hours of Operation

Monday - Friday

9:00am - 5:00pm
US Eastern Time