Cetobacterium somerae Finegold et al.

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #1490 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1490 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
4. Incubate in an anaerobic atmosphere at 37°C for 24 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

• Loose screw caps on test tubes in an anaerobic chamber
• Loose screw caps on test tubes in an activated anaerobic gas pack jar
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

Always use freshly prepared anaerobic medium.  If there is any question about the anaerobic condition of the medium, the medium can be reduced with the addition of 1.5% cysteine (2.0 ml per 100 ml of medium). 

Brucella agar can also be used for growth.

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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