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Pseudogymnoascus destructans (Blehert et Gargas) Minnis et Lindner

MYA-4855

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An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category
Fungi
Classification
Leotiomycetes, Thelebolales, Thelebolaceae
Strain designation
20631-21
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Wing skin of little brown bat, Myotis lucifugus, from Williams Hotel Mine in Ulster County; isolated February 2008
Geographical isolation
United States; New York
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Emerging infectious disease research
Preceptrol
No

Characteristics

Morphology
On SDA at 10°C after 38 days, colonies white to grayish white, raised, dense velvety texture, slow growing, colony diameter after 38 days 1.8-2.3 cm. Production of light to dark brown exudate. Reverse buff to tan. Hyphae guttulate, 2.25 µm thick. Conidiophores with whorls of 2-6 verticils. Conidia formed singly and in short chains of 2-5, smooth, amygdaliform to allantoids with apical scar, containing oil droplets, 6-10.5 µm x 3-4.5 µm.
Comments
Psychrophilic fungus.
Pathogen causing bat white-nose syndrome.
Genome sequencing strain (The Broad Institute, USA).
Individuals that have contact with G. destructans should observe a personal quarantine and not enter bat congregation sites during active work and for 7 days after last contact with the fungus. Lab processes should be managed to avoid inadvertent contact with culture materials. Clothing and other materials brought into the laboratory should be segregated from those transported to any potential bat congregation sites.
The current accepted name of Geomyces destructans is Pseudogymnoascus destructans (Blehert et Gargas) Minnis et Lindner.

Handling information

Medium
Temperature
4-10°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.

  1. To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.
  2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 50 µL (or 2 to 3 agar cubes) of the content onto a plate or broth with medium recommended.
  3. Incubate the inoculum/strain at the temperature and conditions recommended.
  4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 3 to 4 days of incubation. However, the time necessary for significant growth will vary from strain to strain. 

 

Handling notes
Type strain of the species; Pathogen causing bat white-nose syndrome; Psychrophilic fungus; Genome sequencing strain (The Broad Institute, USA); Individuals that have contacted with G. destructans should observe a personal quarantine and not enter bat congregation sites during active work and for 7 days after last contact with the fungus. Lab processes should be managed to avoid inadvertent contact with culture materials, and clothing and other materials brought into the laboratory should be segregated from those transported to any potential bat congregation sites.

Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.

Quality control specifications

Sequenced data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

AAGTATGGAGCAATCTCAATGATTCTTCCGTGGCGACACTTATGGAAGCCTTTGCAGCCCCGCAAGGGGTGGGGAGCACGACTGTAAATAAGTCTCCCTTCATGCAAGTCAGCACCCGCTGGCAACACGATCGAATTGACGGGGACGTCCTAAAGCCTACAACACCAACCCGCCCGGGAAACCGAGGCGGGGGCCCGTGCTAACTCCACGGGGTGGTAAAAGAGTGTATGGATACTCCCTCTGGGGAACTATGGATAATCCGCAGCGAAGACCCTAAGTAGCGCTAGCTATACGGGTAACGTTCACAGACTAAGTGGTTGTGGGTGGAGCCTAGCTCTGCTTAAGATATAGTCGGGCCCTACGTGAAAGCGCAGGGGTGAGTCGCTACGAACTCGAAACCGTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTAGTCGCCCGGGTTGCCGCAAGGCCTCCCGGGTAACCTACCACCCTTTGTTTATTACACTTTGTTGCTTTGGCAGGCCTGCCCTCGGGCTGCTGGCTCCGGCCGGCGAGCGCTTGCCAGAGGACTAAACTCTGTTTGTCTATACTGTCTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCCCCGCCGACCCGGCGGGCCCTAAAGTCAGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCCGGAGGTCCGGTCGTGTGCTTGCCAGCAACCCCCAATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAACAGCTCAAATTTGAAATCTGGCCTCACGGTCCGAGTTGTAATTTGTAGAGGATGCTTCGAGCATGGTCTGGCCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGCGGCCAGGTGCCTACGCTCATGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTTGCGCGCGGCCGATCATCCGGTGTTCTCACCGGTGCACTCGGCCGTGCTCAGGCCAGCATCGGTTTTGGCGGCTGGATAAAGGCCCTAGGAATGTAGCTCCTCTCGGGGAGTGTTATAGTCTAGGGTGCAATGCAGCCTGCTGGGACCGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGGC

Verification method
Whole-genome Sequencing

History

Synonyms
Geomyces destructans Blehert et Gargas
Depositors
DS Blehert
Chain of custody
ATCC <-- DS Blehert
Type of isolate
Animal
Year of origin
2008
Cross references
GenBank KC171349 ITS including 5.8S rRNA gene
GenBank KC171321 D1/D2 region of 28S rRNA gene
GenBank EU884921 Geomyces destructans isolate 20631-21 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1 and 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence.
GenBank FJ231098 Geomyces destructans isolate 20631-21 18S small subunit ribosomal RNA gene, partial sequence.
GenBank AEFC00000000 Pseudogymnoascus destructans 20631-21, whole genome shotgun sequencing project
GenBank LAJJ00000000 Pseudogymnoascus destructans strain 20631-21, whole genome shotgun sequencing project

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Gargas A, et al. Geomyces destructans sp. nov. associated with bat white-nose syndrome. Mycotaxon 108: 147-154, 2009.

Lorch JM, et al. Experimental infection of bats with Geomyces destructans causes white-nose syndrome. Nature 480: 376-378, 2011. PubMed: 22031324

Blehert DS, et al. Bat white-nose syndrome: an emerging fungal pathogen? Science 323(5911): 227, 2009. PubMed: 18974316

Chaturvedi V, et al. Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS). PLoS One 5(5): e10783, 2010. PubMed: 20520731

Minnis AM, Lindner DL. Phylogenetic evaluation of Geomyces and allies reveals no close relatives of Pseudogymnoascus destructans, comb. nov., in bat hibernacula of eastern North America. Fungal Biol 117(9): 638-649, 2013. PubMed: 24012303

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