Biopesticide Registration Program

Ensure Compliance with EPA Guidelines

Program Overview

The Biopesticide Registration Program (BRP) was created by ATCC to provide prospective biopesticide registrants with a streamlined service that ensures compliance with EPA guidelines 885.1100 and 885.1200.

  • Improve Efficiency
  • Authenticate and Store 
  • Prevent Delays in Approvals
  • Save Time and Money

How it Works

ATCC testing and storage procedures draw from our considerable expertise in the authentication and preservation of microorganisms and their toxins and adhere to EPA guidelines for handling microbial pesticides.

Biorepository Management Workflow

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Components of Certificate of Analysis
  Bacteria Protozoa Fungi Yeast Virus
Colony Description  
Biochemical result      
Gram stain      
Sterility Test        
Cell and colony morphological conformity


To assist registrants with the long-term storage of material in the BRP, please see below a quick reference guide on the correct cryoprotectant to use based on cell type. This guide is only to be used as a general guideline, for details please refer to the  Cryopreservation Technical Manual.

Cryopreservation Quick-Reference Chart

Cell Type

No. of Cells

Cryoprotective Agent


Bacteria 107/mL Glycerol (10%) -60°C
Bacteriophage 108 pfu/mL Glycerol (10%) -80°C
Fungi (Hyphae) Glycerol (10%) -150°C
Fungi (Spores) 106/mL Glycerol (10%) -80°C 
Yeast 107/mL Glycerol (10%) -150°C
Protozoa 105-107/mL DMSO (5-10%) or Glycerol (10-20%) -150°C


Additional Resources

For additional information, biopesticide registration program fees or to initiate registration of biopesticides contact ATCC BRP or call 800-638-6597.

† Mycelial masses are prepared for freezing of the hyphae of fungi without regard to numbers of cells.