RPMI Medium without Phenol Red without L-Glutamine (ATCC® 30-2602)

Permits and Restrictions

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Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.

RPMI 1640 is a modification of McCoy’s 5A medium that contains a higher concentration of inositol. The medium is commonly used with mammalian myeloma and hybridoma cells. This formulation does not contain phenol red or L-glutamine.


Supplement with 2 mM L-glutamine prior to use. Modified to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L D-glucose, and 1.5 g/L sodium bicarbonate. Note: This reduced level of sodium bicarbonate is intended for use in a 5% COin air environment. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. pH, osmolality, and endotoxin tests are also performed as indicated below. 




7.0 to 7.4


246 to 306 mOsm/kg H2O

Sterility Testing



≤ 0.25 EU/mL



Growth Promotion


*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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