GeneXPlus Transfection Reagent (ATCC® ACS-4004)

Product Format 1 mL
Storage Conditions -20°C
Applications Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266). A complete list of cell line-specific protocols can be found here.

This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.


GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

Intended Use

This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use.

Biosafety Level N/A
Introduction GeneXPlus Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells.
Quality Control Specifications

Physical characterization of reagent components is performed using mass spectrophotometry and nuclear magnetic resonance (NMR) for identification and determination of purity. GeneXPlus is tested for performance using a luciferase reporter assay in HEK293 cells.



Physical Characterization


Visual Inspection


Functional Assay

>75 ng luciferase/well


2.5 mg/mL in 80% ethanol

*Please consult the Certificate of Analysis for lot-specific test results.

Basic Documentation
Other Documentation
  1. GeneXPlus transfection reagent
    Date Updated: 3/27/2014
  2. HEK293T/17 SF cells are clumping
    No. Small aggregates are easily dispersed with minimal pipetting and do not affect the performance of the cell line.
    Date Updated: 3/27/2014
  3. Replenishing medium for HEK293T/17 SF cells
    Yes. Incomplete media changes affect the cell viability and recovery. Spent medium should be completely removed and replaced with fresh medium every 2 to 3 days.
    Date Updated: 3/27/2014
  4. Media volume for HEK293T/17 SF cells
    For protein expression, it is important to have adequate shaking in the culture vessel. We usually add culture medium to 20% volume of a shaker flask.
    Date Updated: 3/27/2014