AKR1.G.1.OUAR.1.26 (ATCC® TIB-232)

Organism: Mus musculus, mouse  /  Disease: lymphoma

Organism Mus musculus, mouse
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain AKR/J
Applications
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for fusion studies and as a host for the transfection of cloned genes.
Antigen Expression
Thy-1; L3T4; Lyt-2; HSA
Genes Expressed
Thy-1; L3T4; Lyt-2; HSA
Comments
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for fusion studies and as a host for the transfection of cloned genes.
The cells have the surface antigen properties of cortical thymocytes.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
References

. . Immunogenetics 10: 261-271, 1980.

Basic Documentation
References

. . Immunogenetics 10: 261-271, 1980.