LTR228 [LTR 228-TG, derivative of WIL-2NS (ATCC CRL-8155)] (ATCC® HB-8502)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme  / 

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Organism Homo sapiens, human
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Age 5 years
Gender male
Ethnicity Caucasian
Applications
The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.
Karyotype modal number = 48; Y present
Clinical Data
male
Caucasian
5 years
Genes Expressed
immunoglobulin; monoclonal antibody (small amounts)
Comments
The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.
The cells have a high cloning efficiency in soft agar and by limiting dilution.
Complete Growth Medium Iscove's modified Dulbecco's medium with 0.03% L-glutamine and 0.02 mM 6-thioguanine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Isotype IgM; kappa light chain
Population Doubling Time 16 hrs
Name of Depositor Cetus Corp.
References

Raubitschek AR, et al. Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Raubitschek AR, et al. Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986