Synthetic West Nile Virus RNA (ATCC® VR-3198SD)

Product Format: 1 vial/100 reactions

Permits and Restrictions

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Strain Synthetic West Nile Virus RNA
Applications The Synthetic West Nile Virus RNA contains single stranded RNA genetic material designed and synthetically created for use as a genetic surrogate for West Nile Virus, known as molecular standards. Molecular standards are appropriate for use as control material in molecular applications, such as PCR.
Biosafety Level 1
Product Format 1 vial/100 reactions
Storage Conditions 8°C or colder
Comments

Genetic Target: Fragments from the 5'UTR region, anchored capsid protein C gene, capsid protein C gene, membrane glycoprotein precursor prM gene, envelope protein E gene, nonstructural protein NS1, NS2A, NS3, and NS5 genes, and the 3' UTR region of the West Nile Virus genome

Length: ~1500 bp

Format Provided: Dried RNA  

Starting Amount: 1.1 x 108 Copy Numbers (CN)  

Downstream compatibility: Reverse Transcription PCR or other molecular applications  

Special Collection DNA
References

Briese T, Glass W, Lipkin W. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet 355(9215): 1614-1615, 2000 PubMed: 10821368

Eiden M, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest 22(5): 748-753, 2010 PubMed: 20807934

Jimenez-Clavero M, et al. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. J Vet Diagn Invest 18(5): 459-462, 2006 PubMed: 17037613

Lanciotti R, et al. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitos, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38(11): 4066-4071, 2000 PubMed: 11060069

Papin J, Vahrson W, Dittmer D. SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability. J Clin Microbiol 42(4): 1511-1518, 2004 PubMed: 15070997

Parida M, et al. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1): 257-263, 2004 PubMed: 14715762

Shi P, et al. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39(4): 1264-1271, 2001 PubMed: 11283039

Usuku S, Noguchi Y, Takasaki T. Newly developed TaqMan assay to detect West Nile viruses in a wide range of viral strains. Jpn J Infect Dis 57(3): 129-130, 2004 PubMed: 15218227

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. Stability of Norovirus and West Nile standards

    Accelerated stability studies at 45oC have been performed on these samples and no degradation was observed.


    Date Updated: 3/27/2014