Dulbecco's Modified Eagle's Medium (DMEM) without Phenol Red (ATCC® 30-2601)

Permits and Restrictions

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Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.

Dulbecco's Modified Eagle's Medium (DMEM) is a basal medium widely used in mammalian cell culture. The medium is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins. This formulation does not contain phenol red or L-glutamine.

Comments Supplement with 4 mM L-glutamine prior to use.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also tested for the ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. pH, osmolality, and endotoxin tests are also performed as indicated in the Specification Certificate.

Specification Certificate*




7.0 to 7.4


305 to 365 mOsm/kg H2O

Sterility Testing



≤ 0.25 EU/mL



Growth Promotion


*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation