Basic Yeast Synthetic Biology Tool Kit (ATCC® SB-2000)

Permits and Restrictions

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Components
Destination vectors:
pDEST-12y (ATCC SB-1000)
pDEST-23y (ATCC SB-1001)
pDEST-34y (ATCC SB-1002)
pDEST-45y (ATCC SB-1003)

Yeast vectors:
pCarrier 3 (416) (ATCC SB-1004)
pCarrier 7 (426) (ATCC SB-1005)
pCarrier 8 (integ) (ATCC SB-1024)

Yeast selection maker:
pENTR_L1L2_KanMX (ATCC SB-1006)

Yeast constitutive promoters:
pENTR_L4R1_pHOG1 (ATCC SB-1008)
pENTR_L4R1_pHOT1 (ATCC SB-1035)
pENTR_L4R1_pYPD1 (ATCC SB-1041)
pENTR_L4R1_pSKN7 (ATCC SB-1105)

Yeast inducible promoter:
pENTR_L4R1_pGAL1 (ATCC SB-1046)

Entry vectors:
pENTR_L1L2_DONR1 (ATCC SB-1013)
pENTR_L4R1_DONR2 (ATCC SB-1014)
Application A basic tool kit for the construction of gene expression systems and gene circuits in the yeast Saccharomyces cerevisiae
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).

Product Format purified plasmid DNA (2 to 3 µg/vial)
Storage Conditions 4°C or colder
Depositor R Weiss, Massachusetts Institute of Technology
References

Ron Weiss, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ron Weiss, personal communication