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  • Phosphate Buffered Saline Without Calcium Or Magnesium (ATCC® SCRR-2201)

    ATCC® Number: SCRR-2201
    Quantity: 500 mL
    Storage: Store at ambient temperatures, between 15°C and 30°C.
    Applications:

    For washing cells (including embryonic stem cells), for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $13.00 Non-Profit:   $13.00

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  • LB Broth (ATCC® 60-2100)

    ATCC® Number: 60-2100
    Quantity: 1 liter
    Storage: 15°C to 30°C (ambient temperature)
    Applications:

    Sterile liquid is ready to use.
    Luria-Bertani medium for propagation of bacteria for molecular biology applications.

    For-Profit:   $30.00 Non-Profit:   $30.00

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  • Hybri-Care Medium (ATCC® 46-X)

    ATCC® Number: 46-X
    Quantity: 1 Package (prepares 1L of medium)
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines.
    A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 1 Package (prepares 1L of medium)
    Comments:
    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection.

    Ozato, K., et al. J. Immunology 124: 533-540, 1980.
  • TransfeX™ Transfection Reagent (ATCC® ACS-4005)

    ATCC® Number: ACS-4005
    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

    For-Profit:   $225.00 Non-Profit:   $225.00

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality.

  • Fetal Bovine Serum, ES Cell Qualified (ATCC® SCRR-30-2020)

    ATCC® Number: SCRR-30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -70°C in its yellow plastic storage bag. The yellow bag minimizes the effect of light on the serum. If storage at -70°C is not possible, then store at -20°C. Do not store fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications:

    This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state. Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers. The results are compared to a control lot of serum. The serum is triple filtered through 0.1µM filters and each lot of fetal bovine serum is tested for sterility. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States.


    For-Profit:   $424.00 Non-Profit:   $424.00

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    Product Format: frozen 500 mL
    Comments: The results were compared with cells grown with a control lot of serum.
  • F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004)

    ATCC® Number: 30-2004
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments: NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Calf Bovine Serum (ATCC® 30-2031)

    ATCC® Number: 30-2031
    Quantity: 100 mL
    Storage: Store calf bovine serum at -20°C or colder. Do not store frozen calf bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum in aliquots. Calf bovine serum is supplied in PETG plastic bottles.
    Applications: Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $21.00 Non-Profit:   $21.00

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  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

    ATCC® Number: ACS-3002
    Storage: Basal Medium is stored at -20°C. Growth Supplement is stored at 2°C to 8°C.

    For-Profit:   $230.00 Non-Profit:   $230.00

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    Product Format: frozen
    Comments:
    Media components are tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with hESCs and hiPSCs to ensure that pluripotency is maintained as determined by expression of pluripotent markers.
  • Leibovitz's L-15 Medium (ATCC® 30-2008)

    ATCC® Number: 30-2008
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Leibovitz's L-15 Medium contains 2 mM L-glutamine and no sodium bicarbonate.

    For-Profit:   $23.00 Non-Profit:   $23.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This formulation is designed to be used without additional CO2 in the atmosphere of cell cultures.
  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020
    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $50.00 Non-Profit:   $50.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.