Chlamydophila pneumoniae (ATCC® VR-1360)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen 1 mL per vial

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Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae Grayston et al.
Agent Chlamydophila pneumoniae
Strain CM-1
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
This isolate was identified as C. pneumoniae by culture characteristics, morphology of inclusion bodies, and reaction with monoclonal antibodies by depositor. Item isolated on cycloheximide-treated HeLa 229 cells, and found to be serologically identical to the reference strain TW183 by micro-immunofluorescent test.
Effect on Host
Intracellular glycogen negative inclusion bodies visualized by fluorescent staining
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-95% confluent. Vortex material with 1mm glass beads to disrupt cells. Remove cell growth medium and inoculate with disrupted material. Centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Incubation: 3 days
Effect on Host
Intracellular glycogen negative inclusion bodies visualized by fluorescent staining
Name of Depositor KH Wong, Centers for Disease Control and Prevention
Special Collection NCRR Contract
Source Sputum of pneumonia patient, Georgia
References

Black CM, et al. Antigenic variation among strains of Chlamydia pneumoniae. J. Clin. Microbiol. 29: 1312-1316, 1991. PubMed: 1885727

Cross References

Nucleotide (GenBank) : U44033 Chlamydia pneumoniae putative ribonuclease P RNA gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation