Parachlamydia acanthamoebae Everett et al. (ATCC® VR-1476)

Product Format: frozen

Agent Parachlamydia acanthamoebae Everett et al.
Strain Bn9
Biosafety Level 2
Isolation
parasitized amoebae from the nasal mucosa of a human female, Germany
Product Format frozen
Storage Conditions -70°C or colder
Comments
-70°C or lower
Effect on Host
Eventually causes death of host cells
Induces change in appearance of amoebae away from pleomorphic form to cyst-like form.  Eventually causes death of the host cells.
Recommended Host
Acanthamoeba castellanii (ATCC 50739)
Acanthamoeba castellanii; other Acanthamoeba sp.
Growth Conditions
Temperature: 30°C
Incubation: 3-4 days at 30°C in Acanthamoeba castellanii (ATCC 50739)
Cryopreservation
Storage temperature: -70C or lower
Effect on Host
Eventually causes death of host cells
Induces change in appearance of amoebae away from pleomorphic form to cyst-like form.  Eventually causes death of the host cells.
Name of Depositor R Michel
Chain of Custody
ATCC <-- R Michel <-- I Weishaar
Source
parasitized amoebae from the nasal mucosa of a human female, Germany
References

Michel R, Hauroder-Philippczyk B. Acanthamoeba from human nasal mucusa infected with an obligate intracellular parasite. Eur. J. Protistol. 30: 104-110, 1994.

Amann R, et al. Obligate intracellular bacterial parasites of Acanthamoebae related to Chlamydia spp.. Appl. Environ. Microbiol. 63: 115-121, 1997. PubMed: 8979345

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462