Rickettsia parkeri (ATCC® VR-1637)

Classification: Rickettsiaceae, Rickettsieae  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Rickettsiaceae, Rickettsieae
Agent Rickettsia parkeri
Strain Maculatum C
Biosafety Level 3
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host CPE, rounding
Recommended Host Vero (ATCC® CCL-81™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of agent (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb by centrifugation at 800 x g for 30 minutes. End adsorption by adding virus growth medium.
Incubation: 7-14 days
Effect on Host CPE, rounding
Name of Depositor GA Dasch, Naval Medical Research Center
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation