Orientia chuto (ATCC® VR-1744)

Classification: Rickettsiaceae, Orientia  /  Product Format: frozen

Permits and Restrictions

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Classification Rickettsiaceae, Orientia
Agent Orientia chuto
Strain Dubai
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions  -70°C or colder
New Taxon.
It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects.
Effect on Host
No CPE produced
Recommended Host
Vero cells (ATCC CCL-81)

Production Host:  Vero cells (ATCC® CCL-81™)

Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and inoculate with an appropriate volume of Rickettsiae (e.g. 1 mL per 25 cm2) diluted in agent growth medium to provide an appropriate MOI (e.g. MOI of 50 for 50 Rickettsiae/ cell). Adsorb 1 hour at 800 x g at 20?C in a low speed centrifuge in closed buckets. End adsorption by adding virus growth medium to culture(s).
Incubation: Incubate infected culture(s) for 7-14 days at 35°C in a humidified 5% CO2 atmosphere.
Effect on Host
No CPE produced
Name of Depositor L Izzard
Clinical female patient with acute scrub typhus infection, Dubai, United Arad Emirates, 2006.

Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Micro. 48(12): 4404-4409, 2010.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation