L-NGC-5HT2 (ATCC® CRL-10287)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1 (SV-40 Transformed African Green Monkey)
Strain C3H/An
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of L-M(TK-) that is stably transfected with the gene for the human type 2 5-hydroxytryptamine (serotonin) receptor (5HT2).
Receptor Expression
5-hydroxytryptamine (serotonin, 5HT2), expressed
Comments
This line is a derivative of L-M(TK-) that is stably transfected with the gene for the human type 2 5-hydroxytryptamine (serotonin) receptor (5HT2).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor Synaptic Pharmaceutical Corporation
References

Gluchowski C, et al. Use of alpha1C specific compounds to treat benign prostatic hyperplasia. US Patent 5,403,847 dated Apr 4 1995

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gluchowski C, et al. Use of alpha1C specific compounds to treat benign prostatic hyperplasia. US Patent 5,403,847 dated Apr 4 1995