Ltk-11 (ATCC® CRL-10422)

Organism: Mus musculus, mouse  / 

Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 100 days adult
Gender male
Strain C3H/An
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human 5HT1Dbeta receptor.
Clinical Data
male
Receptor Expression
5-hydroxytryptamine (serotonin) 1D beta (5HT1Dbeta)
Comments
This line is a derivative of L-M(TK-) (see ATCC CCL-1.3) that is stably transfected with the gene for the human 5HT1Dbeta receptor.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor Synaptic Pharmaceutical Corporation
References

Konkel M, et al. Compounds specific for the human .alpha.1d adrenergic receptor and uses thereof. U.S. Patent 6,706,716 dated Mar 16 2004

Basic Documentation
References

Konkel M, et al. Compounds specific for the human .alpha.1d adrenergic receptor and uses thereof. U.S. Patent 6,706,716 dated Mar 16 2004