RL (ATCC® CRL-2261)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: ascites  /  Disease: non-Hodgkin's lymphoma

Organism Homo sapiens, human
Tissue ascites
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease non-Hodgkin's lymphoma
Age 52 years
Gender male
Ethnicity Caucasian
Karyotype the cells possess a 14:18 translocation
Derivation
RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Clinical Data
52 years
Caucasian
male
Antigen Expression
CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
Genes Expressed
immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Cellular Products
immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Comments
Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.

RL cells have been reported to be Epstein-Barr virus genome negative.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 and 1 x 106.


Medium Renewal: 2 to 3 times a week.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9,10
TPOX: 9,11
vWA: 19,20
Isotype IgM; lambda light chain
Name of Depositor M Beckwith
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

Basic Documentation
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723