OML, clone 13C (ATCC® CRL-2312)

Organism: Aotus trivirgatus, monkey, Owl  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme  / 

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Organism Aotus trivirgatus, monkey, Owl
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, The cells grow in dense clumps.
Biosafety Level 2 Cells contain Herpesvirus
Age adult
Gender female
Applications
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Karyotype The cells exhibit a karyotype consistent with the species Aotus trivirgatus; the chromosome count was 54 with an XX sex chromosome constitution., Analysis revealed a G-banding pattern of the type designated karyotype I.
Derivation
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
female
Antigen Expression
CD20 +
Genes Expressed
CD20 +
Comments
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The resulting cells were cloned by limiting dilution.
CITES Permit
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml.
Maintain cultures at cell concentrations between 5 x 10(5) and 2 x 10(6) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 24 hrs
Name of Depositor JG Scammell
Year of Origin 1995
References

Scammell JG, et al. An EBV-transformed owl monkey B-lymphocyte cell line. In Vitro Cell. Dev. Biol. Anim. 33: 88-91, 1997. PubMed: 9081215

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Scammell JG, et al. An EBV-transformed owl monkey B-lymphocyte cell line. In Vitro Cell. Dev. Biol. Anim. 33: 88-91, 1997. PubMed: 9081215