HCC2157 BL (ATCC® CRL-2341)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme  /  Disease: Carcinoma

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Organism Homo sapiens, human
Cell Type lymphoblast B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, multicell aggregates
Biosafety Level 2 Cells contain Herpes Virus (Epstein-Barr Virus)
Disease Carcinoma
Age 48 years adult
Gender female
Ethnicity Black
Applications
HCC2157 was initiated from a 48 year old black female with primary breast ductal carcinoma, and is available as ATCC CRL-2340.
Storage Conditions liquid nitrogen vapor phase
Derivation
HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157.
HCC2157 was initiated from a 48 year old black female with primary breast ductal carcinoma, and is available as ATCC CRL-2340.
Clinical Data
HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157.
HCC2157 was initiated from a 48 year old black female with primary breast ductal carcinoma, and is available as ATCC CRL-2340.
female
Black
48 years adult
Comments
HCC2157 BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC2157.
HCC2157 was initiated from a 48 year old black female with primary breast ductal carcinoma, and is available as ATCC CRL-2340.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 11,12
D5S818: 12,13
D7S820: 10,12
THO1: 8,9
TPOX: 9,11
vWA: 14,18
Name of Depositor AF Gazdar, AK Virmani
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771