SJSA-1 (ATCC® CRL-2098)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: osteosarcoma; multipotential sarcoma

Organism Homo sapiens, human
Tissue
bone
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease osteosarcoma; multipotential sarcoma
Age 19 years adult
Gender male
Ethnicity Black
Storage Conditions liquid nitrogen vapor temperature
Karyotype 44XY; -1, -4, -7, -9, -11, -14, -15, +del(1)(q32;q42),+5p+, +der(7)t(4;HSR;7)(4qter-4q12::HSR::7p22--7qter), +9p+ and markers; amplified gli sequences were contained in the HSR in derivative No. 7 chromosomes
Derivation
The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur.
Clinical Data
male
19 years
Black
Comments
There is amplification of the gene which encodes the p53 associated protein, MDM2.

SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Two to three times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor DN Shapiro
Year of Origin 1982
References

Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305

Basic Documentation
References

Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305