WEHI-279 (ATCC® CRL-1704)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Tissue: bone marrow  /  Disease: lymphoma

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Organism Mus musculus, mouse
Tissue bone marrow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, moderate to large multi-cell aggregates
Biosafety Level 1
Disease lymphoma
Strain NZC
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed Expresses immunoglobulin on its surface only (surface IgM, kappa). It does not secrete any detectable immunoglobulin.
Comments

Expresses immunoglobulin on its surface only (surface IgM, kappa). It does not secrete any detectable immunoglobulin.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 cells/mL.
Frozen cells may take longer than 2-3 days in complete culture medium to successfully recover from the frozen state.
Medium Renewal: 2 to 3 times per week
Cryopreservation Complete growth medium, 95%; DMSO, 5%
Culture Conditions Temperature: 37°C
Atmosphere: 5% CO2 in air atmosphere.
Isotype IgM (surface); kappa light chain
Name of Depositor NL Warner, LL Lanier
References

Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803