TF-1 (ATCC® CRL-2003)

Organism: Homo sapiens, human  /  Cell Type: erythroblast  /  Tissue: bone marrow  /  Disease: erythroleukemia

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type erythroblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease erythroleukemia
Age 35 years
Gender male
Ethnicity Japanese
Applications
This cell line provides a good system for investigating the proliferation and differentiation of myeloid progenitor cells.
Storage Conditions liquid nitrogen vapor phase
Derivation
The TF-1 cell line was established by T. Kitamura, et al. in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. ­
Clinical Data
male
Japanese
35 years

Comments

The cells are completely dependent on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. 

The cells DO NOT RESPOND to interleukin 5 (IL-5). TF-1 cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9),Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). TF-1 cells do not express glycophorin A or carbonyl anhydrase I. 

The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells. 

The TF-1 cell line is unique because of its responsiveness to multiple cytokines.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2 ng/ml recombinant human GM-CSF; fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 104 viable cells/mL. Do not allow the cell concentration to reach 7 X 10cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cultures between 3 X 104 and 5 X 105 viable cells/mL
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 13,14
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Population Doubling Time 22 hours
Name of Depositor T Kitamura
Year of Origin 1987
References

Kitamura T, et al. IL-1 up-regulates the expression of cytokine receptors on a factor- dependent human hemopoietic cell line, TF-1. Int. Immunol. 3: 571-577, 1991. PubMed: 1832294

Kitamura T, et al. Identification and analysis of human erythropoietin receptors on a factor-dependent cell line, TF-1. Blood 73: 375-380, 1989. PubMed: 2537111

Kitamura T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334, 1989. PubMed: 2663885

Borellini F, Glazer RI. Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1. J. Biol. Chem. 268: 7923-7928, 1993. PubMed: 8463313

Schulte T, et al. Molecular characterization and functional analysis of murine interleukin 4 receptor allotypes. J. Exp. Med. 186: 1419-1429, 1997. PubMed: 9348299

Mueller CG, et al. Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells. J. Exp. Med. 186: 655-663, 1997. PubMed: 9271581

Limon A, et al. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells. Blood 90: 3316-3321, 1997. PubMed: 9345013

Hu X, et al. Prolonged activation of the mitogen-activated protein kinase pathway is required for macrophage-like differentiation of a human myeloid leukemic cell line. Cell Growth Differ. 11: 191-200, 2000. PubMed: 10775036

USP Monographs: Sargramostim. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kitamura T, et al. IL-1 up-regulates the expression of cytokine receptors on a factor- dependent human hemopoietic cell line, TF-1. Int. Immunol. 3: 571-577, 1991. PubMed: 1832294

Kitamura T, et al. Identification and analysis of human erythropoietin receptors on a factor-dependent cell line, TF-1. Blood 73: 375-380, 1989. PubMed: 2537111

Kitamura T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334, 1989. PubMed: 2663885

Borellini F, Glazer RI. Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1. J. Biol. Chem. 268: 7923-7928, 1993. PubMed: 8463313

Schulte T, et al. Molecular characterization and functional analysis of murine interleukin 4 receptor allotypes. J. Exp. Med. 186: 1419-1429, 1997. PubMed: 9348299

Mueller CG, et al. Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells. J. Exp. Med. 186: 655-663, 1997. PubMed: 9271581

Limon A, et al. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells. Blood 90: 3316-3321, 1997. PubMed: 9345013

Hu X, et al. Prolonged activation of the mitogen-activated protein kinase pathway is required for macrophage-like differentiation of a human myeloid leukemic cell line. Cell Growth Differ. 11: 191-200, 2000. PubMed: 10775036

USP Monographs: Sargramostim. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011