SR-4987 (ATCC® CRL-2028)

Organism: Mus musculus, mouse  /  Cell Type: virus transformed  /  Disease: Leukemia

Organism Mus musculus, mouse
Cell Type virus transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2
Disease Leukemia
Age 5 months
Gender female
Strain BDF1
Applications
transfection host
Karyotype tetraploid
Derivation
The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
Clinical Data
female
Genes Expressed
macrophage colony stimulating factor (MCSF)
Cellular Products
macrophage colony stimulating factor (MCSF)
Tumorigenic Yes
Effects
Yes, produce sarcomas in syngeneic mice
Comments
The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
The cells produce MCSF, and are highly sensitive to fibroblast growth factor (FGF).
They are poorly clonogenic in soft agar.
Neither interleukin 3 (IL-3) or granulocyte colony stimulating factor (GCSF) were detected in SR-4987 conditioned medium.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove the trypsin.
Add fresh trypsin solution (1 to 2 ml), and allow the culture to sit at room temperature (or at 37C) until the cells begin to detach.
Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time 15 hrs
Name of Depositor G Zaleskis
References

Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506

Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333

Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078

Basic Documentation
References

Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506

Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333

Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078