TF-1.CN5a.1 (ATCC® CRL-2512)

Organism: Homo sapiens, human  /  Cell Type: erythroblast  /  Disease: erythroleukemia

Organism Homo sapiens, human
Cell Type erythroblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain CMV viral sequences
Disease erythroleukemia
Age 35 years
Gender male
Ethnicity Japanese
Applications
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
The cells can be used to access the potency and to study signal transduction of CNTF.
Storage Conditions liquid nitrogen vapor phase
Derivation
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
Clinical Data
male
Japanese
35 years
Receptor Expression
alpha subunit of human ciliary neurotrophic factor (CNTF) (receptor is not expressed by TF-1 cells)
Comments
The cells stably express the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor and CNTF supports the short term proliferation of the cells. Suggested assay conditions are washing the cells with RPMI to remove GM-CSF, then incubation in RPMI 1640 95%, fetal bovine serum 5%, and picogram/ml concentrations of CNTF. The cells maintain responsiveness for at least 193 population doublings in full maintenance medium.
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
TF-1 cells were transfected, using Transfectam, with the gene for the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor ligated to the pCR3.1 vector.
The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene.
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
TF-1.CN5a cells were grown, transiently, in the absence of GM-CSF and with 2 ng/ml CNTF to establish the TF-1.CN5a.1 cell line.
The cells can be used to access the potency and to study signal transduction of CNTF.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 2 ng/ml GM-CSF and 0.4 mg/ml G-418, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Name of Depositor CJ Robinson
Year of Origin 2003