G355-5 (ATCC® CRL-2033)

Organism: Felis catus, cat  /  Cell Type: astrocyte  / 

Permits and Restrictions

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Organism Felis catus, cat
Cell Type astrocyte
Product Format frozen
Morphology glial, astrocyte
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age embryo
Applications
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Derivation
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
Comments
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated fetal bovine serum to a final concentration of 10%
  • Subculturing
    Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
    Medium Renewal: 2 to 3 times per week
    Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution.
    Remove the trypsin, add fresh trypsin (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
    Add fresh medium, aspirate and dispense into new flasks.
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Name of Depositor KJ Dunn
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413