CHLA-01-MED (ATCC® CRL-3021)

Organism: Homo sapiens, human  /  Cell Type: Medulloblastoma  /  Tissue: brain/brain tumor  /  Disease: medulloblastoma

Organism Homo sapiens, human
Tissue
brain/brain tumor
Cell Type Medulloblastoma
Product Format frozen
Morphology rounded
Culture Properties single cells and tight clusters in suspension
Biosafety Level 1
Disease medulloblastoma
Age 8 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human male cell line with a chromosome range of 42∼46. The karyotype contained two consistent chromosome rearrangements, der(17)t(1;17)(q11;q25) and der(18)(17;18)(q11.2;p11.2) and approximately 40% of the examined metaphase spreads contained 11∼16 double minutes.
Images
Derivation

CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. Cells were isolated from the brain tumor resected at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. When placed in medium containing fetal bovine serum the cells attach and spread, but after a period of proliferation they senesce.

A metastatic recurrent medulloblastoma cell line from this patient is available as CHLA-01R-MED (see ATCC CRL-3034)

Clinical Data
CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma.
male
Comments

CHLA-01-MED was established from an 8 year old boy with CNS-disseminated medulloblastoma. Cells were isolated from the brain tumor resected at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption. This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. When placed in medium containing fetal bovine serum the cells attach and spread, but after a period of proliferation they senesce. The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Childrens Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.

A metastatic recurrent medulloblastoma cell line from this patient is available as CHLA-01R-MED (see ATCC CRL-3034)

Complete Growth Medium DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing
Note: Due to large, tight clusters formed by these cells, it may be difficult to accurately measure cell number and viability.
Protocol: Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Add fresh medium every 4 to 7 days (depending on cell density).
Cryopreservation
Freeze medium: fetal bovine serum, 50%; Cell Freezing Medium-DMSO (Sigma, Cat. No. C-6295), 50%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X Y
CSF1PO: 11
D13S317: 8, 14
D16S539: 9, 11
D5S818: 11, 13
D7S820: 10
THO1: 6, 9.3
TPOX: 9, 12
vWA: 16, 17
Name of Depositor Anat Erdreich-Epstein, Saban Research Institute, Childrens Hospital Los Angeles