Human genomic DNA (HCC38) (ATCC® CRL-2314D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Product Format frozen 10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1
Introduction

Cell Line Description:

This cell line was initiated on April 27, 1992 and took 32 months to establish. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.  HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12. DNA from an EBV- transformed lymphoblastoid cell line, HCC38 BL, from the same patient is available as ATCC CRL-2346D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation