CMT-93 (ATCC® CCL-223)

Organism: Mus musculus, mouse  /  Tissue: rectum  /  Disease: polyploid carcinoma

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Organism Mus musculus, mouse
Tissue rectum
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease polyploid carcinoma
Age 19 months
Gender male
Strain C57BL/icrf
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Antigen Expression
H-2b
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

Tested and found negative for ectromelia virus (mousepox).

This cell line is tumorigenic in mice. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.  Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor LM Franks
References

Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978. PubMed: 722371

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978. PubMed: 722371