SW1463 [SW 1463, SW-1463] (ATCC® CCL-234)

Organism: Homo sapiens, human  /  Tissue: rectum  /  Disease: Dukes' type C, colorectal adenocarcinoma

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Organism Homo sapiens, human
Tissue rectum
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type C, colorectal adenocarcinoma
Age 66 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was derived from a grade II mucous producing and grade III solid tumor.
Clinical Data
66 years
Caucasian
female
Antigen Expression
blood type A; Rh +
Genes Expressed
carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.

 

Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Subculture just prior to confluency

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mof complete growth medium and aspirate cells by gently pipetting. 
  5. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.
  7. Incubate cultures at 37°C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week

Culture Conditions
Atmosphere: Air, 100%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16
Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Name of Depositor A Leibovitz
References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501