SNU-C2B (ATCC® CCL-250)

Organism: Homo sapiens, human  /  Disease: colorectal carcinoma

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent, multicell aggregates
Biosafety Level 1
Disease colorectal carcinoma
Age 43 years
Gender female
Ethnicity Asian
Applications
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 46; range = 45 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 70% with polyploidy at 16.4%. The del (3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11;?) and 2 other marker chromosomes are common to all metaphases examined. Other structurally altered chromosomes, if any, occur only sporadically. The t(1;8) and t(1;?) are not found in CCL-250.1. Normal N8 and N11 are absent. The X chromosomes are two copies per cell. The Y chromosome is not detected by Q-banding.
Derivation
This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
Clinical Data
female
Asian
43 years
Antigen Expression
Blood type A; Rh+
Receptor Expression
epidermal growth factor (EGF)
Genes Expressed
carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days,Blood type A; Rh+
Cellular Products
carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

The cells have a high concentration of receptors for EGF.

Note that this line has lost both chromosomes 8 and 11 while SNU-C2A (ATCC CCL-250.1) has lost only chromosome 11.

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  4. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  5. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  6. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11, 14
D13S317: 9, 10, 11
D16S539: 8, 9
D5S818: 8, 9
D7S820: 13, 16
THO1: 9
TPOX: 8
vWA: 15, 18, 19
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar
Passage History
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
Year of Origin 1982
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Basic Documentation
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249