CCD-112CoN (ATCC® CRL-1541)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: normal

Organism Homo sapiens, human
Tissue
colon
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 22 weeks gestation
Gender female
Karyotype 46, XX; modal number = 46; polyploidy at 5.5%; inv(9)(p13q13)
Clinical Data
female
Comments
The line begins to senesce at population doubling level (PDL) = 76.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,13
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,9
THO1: 6,7
TPOX: 8
vWA: 16,18
Population Doubling Capacity The line begins to senesce at population doubling level (PDL) = 76.
Name of Depositor ATCC