SIRC [Statens Seruminstitut Rabbit Cornea] (ATCC® CCL-60)

Organism: Oryctolagus cuniculus, rabbit  /  Tissue: cornea  / 

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Organism Oryctolagus cuniculus, rabbit
Tissue cornea
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Applications This cell line is suitable for primary isolation of rubella virus. The early appearance of distinct cytopathic changes makes this cell line highly suitable for both the propagation and quantitation of rubella virus.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 66; range = 51 to 90
The modal number (66) does not permit sexing of donor cytologically. Most cells contain one long, unpaired, submetacentric chromosome and 2 to 3 small telocentric chromosomes with satellites. Polyploidy was observed in 5/200 metaphase cells.
Derivation
The SIRC cell line was derived by M. Volkert of the Staatens Seruminstitut, Copenhagen, Denmark, from the cornea of a normal rabbit in 1957.
Virus Susceptibility Rubella virus , Rubella virus
Comments

In 1965, J. Leerhoy found this line to be susceptible to rubella virus. C.A. Phillips, et al. confirmed the above findings and demonstrated the suitability of the cell line for primary isolation of rubella virus.

  

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor J Leerhoy
Passage History
Little is recorded relating to the history of this cell line for approximately the first 400 passages.
Year of Origin 1957
References

Leerhoy J. Cytopathic effect of rubella virus in a rabbit-cornea cell line. Science 149: 633-634, 1965. PubMed: 14331182

Phillips CA, et al. Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 122: 783-786, 1966. PubMed: 5918951

Rhim JS, et al. Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 125: 1271-1274, 1967. PubMed: 6042441

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Leerhoy J. Cytopathic effect of rubella virus in a rabbit-cornea cell line. Science 149: 633-634, 1965. PubMed: 14331182

Phillips CA, et al. Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 122: 783-786, 1966. PubMed: 5918951

Rhim JS, et al. Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 125: 1271-1274, 1967. PubMed: 6042441

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741