Fugu eye (ATCC® CRL-2641)

Organism: Fugu rubripes, torafugu  / 

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Organism Fugu rubripes, torafugu
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age juvenile juvenile
Applications
The Fugu eye cell line was established in 1996 from normal eye tissue.
The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
Derivation
The Fugu eye cell line was established in 1996 from normal eye tissue.
Comments
The Fugu eye cell line was established in 1996 from normal eye tissue.
Fugu cells maintain an unusually compact genome size with small introns.
They are telomerase positive.
The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
Complete Growth Medium These cells are grown in
  • 67.5% DMEM HG without sodium bicarbonate (Invitrogen Cat no. 12100)
  • 25% L-15 (ATCC Cat no.30-2008)
  • 7.5% Ham?s F12 without sodium bicarbonate (Invitrogen Cat no. 21700)

Supplemented with:
  • 0.5 g/L sodium bicarbonate
  • 15 mM HEPES
  • 0.01 mg/ml bovine insulin
  • 0.05 mM 2-mercaptoethanol
  • 1.0 mM L-glutamine
  • 0.05 mM non-essential amino acids
  • 10 ng/ml basic human recombinant FGF
  • 50 ng/ml mouse EGF (Do not filter)
  • 5 to 7.5% heat-inactivated fetal bovine serum

Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 21 to 23°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
  7. Incubate cultures at 21 to 23°C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended; cell density must be high.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 22.0°C; (Max. 23.0 °C, Min. 21.0°C)
Population Doubling Time 2.5 days
Name of Depositor DW Barnes
Year of Origin 1996
References

Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286