IA-XsSBR (ATCC® CRL-1677)

Organism: Rattus norvegicus, rat  /  Cell Type: Epithelial,Epithelial-like  /  Disease: adenocarcinoma; radiation (X-ray) induced

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Organism Rattus norvegicus, rat
Cell Type Epithelial,Epithelial-like
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma; radiation (X-ray) induced
Age adult
Gender male
Strain outbred Holtzman
Applications
The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat.
The cells are sensitive to lysis by peripheral blood lymphoid cells from Fischer F344 rats having colon and pancreatic tumors induced by 1,2-dimethylhydrazine or 7,12-dimethylbenz(a)anthracene.
Derivation
The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat. The cells are sensitive to lysis by peripheral blood lymphoid cells from Fischer F344 rats having colon and pancreatic tumors induced by 1,2-dimethylhydrazine or 7,12-dimethylbenz(a)anthracene.
Clinical Data
The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat.
male
Comments
The IA-XsSBR epithelial-like cell line is a radiation (X-ray) induced small intestine adenocarcinoma derived from an adult male Holtzman rat. The cells are sensitive to lysis by peripheral blood lymphoid cells from Fischer F344 rats having colon and pancreatic tumors induced by 1,2-dimethylhydrazine or 7,12-dimethylbenz(a)anthracene.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor RH Stevens
References

Stevens RH, et al. Lymphocyte cytotoxicity in X-irradiation-induced adenocarcinoma of the rat small bowel. I. Measurement of target cell destruction by release of radioiodinated membrane proteins: brief communication. J. Natl. Cancer Inst. 59: 1315-1319, 1977. PubMed: 904002

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Stevens RH, et al. Lymphocyte cytotoxicity in X-irradiation-induced adenocarcinoma of the rat small bowel. I. Measurement of target cell destruction by release of radioiodinated membrane proteins: brief communication. J. Natl. Cancer Inst. 59: 1315-1319, 1977. PubMed: 904002