EGC/PK060399egfr (ATCC® CRL-2690)

Organism: Rattus rattus, rat  /  Cell Type: enteroglial  / 

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Organism Rattus rattus, rat
Cell Type enteroglial
Product Format frozen
Morphology glial
Culture Properties adherent
Biosafety Level 1
Age adult adult
Gender male
Strain Sprague-Dawley
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
glial fibrillary acidic protein (GFAP); S-100 protein and vimentin
Comments
The cells display robust glial fibrillary acidic protein (GFAP), S-100 and vimentin immunoreactivities, but no Thy-1.1, desmin, smooth muscle alpha-actin or C3 complement receptor immunoreactivity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor A Ruhl
Year of Origin March 6, 1999
References

Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131

The EGC/PK060399egfr enteroglial cell line was established on March 6, 1999 from adult rat myenteric plexus from the jejunum. Purified primaries were transfected with supernants from the virus producing cell line GPE-egfr-neu. GPE-egfr-neu cells contain the chimeric gene egfr/neu and the neomycin-resistance gene neo under the control of the long-terminal repeats of the mouse Moloney leukaemia virus. The egfr/neu gene codes for the extracellular domain of the human EGF receptor and the intracellular tyrosine kinase domain of the proto-oncogene c-neu.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131

The EGC/PK060399egfr enteroglial cell line was established on March 6, 1999 from adult rat myenteric plexus from the jejunum. Purified primaries were transfected with supernants from the virus producing cell line GPE-egfr-neu. GPE-egfr-neu cells contain the chimeric gene egfr/neu and the neomycin-resistance gene neo under the control of the long-terminal repeats of the mouse Moloney leukaemia virus. The egfr/neu gene codes for the extracellular domain of the human EGF receptor and the intracellular tyrosine kinase domain of the proto-oncogene c-neu.