NCTC clone 3526 [derivative of LLC-MK2] (ATCC® CCL-7.2)

Organism: Macaca mulatta, monkey, rhesus  / 

Organism Macaca mulatta, monkey, rhesus
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Applications
The cells are positive for keratin by immunoperoxidase staining.
Genes Expressed
The cells are positive for keratin by immunoperoxidase staining.
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
Grows in chemically defined medium.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium NCTC 135 medium, 100%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Name of Depositor VJ Evans
References

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

J. Natl. Cancer Inst. 29: 749, 1962.

Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902

. . Am. J. Hyg. 70: 275, 1959.

Basic Documentation
References

Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

J. Natl. Cancer Inst. 29: 749, 1962.

Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902

. . Am. J. Hyg. 70: 275, 1959.