ESK-4 (ATCC® CL-184)

Organism: Sus scrofa, pig  / 

Organism Sus scrofa, pig
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Age embryo
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype normal female; diploid
Clinical Data
female
Virus Susceptibility Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA, remove trypsin and let the flask sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor Syntex (USA), Inc.
References

Bordt DE, Thomas PC. Diploid porcine embryonic cell strains, cultures produced therefrom, and use of said cultures for production of vaccines. US Patent 4,070,453 dated Jan 24 1978

Basic Documentation
References

Bordt DE, Thomas PC. Diploid porcine embryonic cell strains, cultures produced therefrom, and use of said cultures for production of vaccines. US Patent 4,070,453 dated Jan 24 1978