BHK570 (ATCC® CRL-10314)

Organism: Mesocricetus auratus, hamster, Syrian golden  /  Cell Type: fibroblast  /  Tissue: kidney  / 

Organism Mesocricetus auratus, hamster, Syrian golden
Tissue kidney
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Applications Used in producing a mammalian G protein coupled glutamate receptor [U.S. Pat. 5,385,831].
The cells are useful as hosts for transfection studies.
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Comments
The BHK-570 cell line is a baby hamster kidney cell line that is thymidine kinase deficient (TK-) RefHorn IR, et al. Molecular analysis of ligant binding to the second cluster of complement-type repeats of the low density lipoprotein receptor-related protein. J. Biol. Chem. 272: 13608-13613, 1997. PubMed: 9153209. The cells are useful as hosts for transfection studies. Used in producing a mammalian G protein coupled glutamate receptor [U.S. Pat. 5,385,831].
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor Univ. Washington, ZymoGenetics, Inc.
References

Mulvihill ER, et al. Method for producing a mammalian G protein copuled glutamate receptor. US Patent 5,385,831 dated Jan 31 1995

Basic Documentation
Other Documentation
References

Mulvihill ER, et al. Method for producing a mammalian G protein copuled glutamate receptor. US Patent 5,385,831 dated Jan 31 1995