NRK-49F (ATCC® CRL-1570)

Organism: Rattus norvegicus, rat  /  Tissue: kidney  / 

Organism Rattus norvegicus, rat
Tissue
kidney
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Applications This cell line is a suitable transfection host.
Receptor Expression
epidermal growth factor (EGF); multiplication stimulating activity (MSA)
Comments
NRK-49F cells are contact inhibited and very sensitive to transformation by chemicals and viruses.
Cultures should be subcultured before they become confluent.
If allowed to become confluent, the cells will transform.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor JE DeLarco
References

de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450

Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742

Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610

Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987

Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

Centrella M, et al. Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513

Basic Documentation
References

de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450

Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742

Sachsenmeier KF, et al. Transforming growth factor-beta1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J. Biol. Chem. 271: 5-8, 1996. PubMed: 8550610

Schultz-Cherry S, Hinshaw VS. Influenza virus neuraminidase activates latent transforming growth factor beta. J. Virol. 70: 8624-8629, 1996. PubMed: 8970987

Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

Centrella M, et al. Rapid flux in transforming growth factor-beta receptors on bone cells. J. Biol. Chem. 271: 18616-18622, 1996. PubMed: 8702513