Super Dome (ATCC® CRL-2286)

Organism: Canis familiaris  /  Cell Type: Epithelial,Epithelial-like  / 

Permits and Restrictions

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Organism Canis familiaris
Cell Type Epithelial,Epithelial-like
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender female
Applications
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Storage Conditions liquid nitrogen vapor temperature
Derivation
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Clinical Data
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
female
Comments
Super Dome is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
Super Dome forms domes that are approximately 5 times the area of MDCK.
For dome formation, the cells should be fed twice a day by a complete medium change.
Complete Growth Medium These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor RJ Klebe
References

Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715