XLK-WG (ATCC® CRL-2527)

Organism: Xenopus laevis, frog, South African clawed  / 

Organism Xenopus laevis, frog, South African clawed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult adult
Gender female
Applications
The coiled bodies can be identified with mAb H1 and affinity-purified rabbit serum C236 against Xenopus coilin.
This cell line is used for the study of coiled bodies.
XLK-WG cells are useful for making heterokaryons with mammalian cells because they can be maintained at 32C.
Storage Conditions liquid nitrogen vapor phase
Derivation
The XLK-WG cell line was initiated in April 29, 1996 by primary cultivation of normal kidneys from an adult female toad by Zheng'an Wu and Joseph G. Gall.
The nuclei of the cells display prominent nucleoli and Cajal bodies (coiled bodies, CBs). The coiled bodies can be identified with mAb H1 and affinity-purified rabbit serum C236 against Xenopus coilin.
Clinical Data
The XLK-WG cell line was initiated in April 29, 1996 by primary cultivation of normal kidneys from an adult female toad by Zheng'an Wu and Joseph G. Gall.
female
Comments
The XLK-WG cell line was initiated in April 29, 1996 by primary cultivation of normal kidneys from an adult female toad by Zheng'an Wu and Joseph G. Gall.
The cell line contains typical mononucleated cells as well as multinucleated cells.
The nuclei of the cells display prominent nucleoli and Cajal bodies (coiled bodies, CBs). The coiled bodies can be identified with mAb H1 and affinity-purified rabbit serum C236 against Xenopus coilin.
This cell line is used for the study of coiled bodies.
XLK-WG cells are useful for making heterokaryons with mammalian cells because they can be maintained at 32C.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 60%; distilled water, 20%; fetal bovine serum, 20%
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 32.0°C
Name of Depositor Z Wu, JG Gall
Year of Origin April 29, 1996
References

Martin OC, et al. Xgrip109: a gamma tubulin-associated protein with an essential role in gamma tubulin ring complex (gammaTuRC) assembly and centrosome function. J. Cell Biol. 141: 675-687, 1998. PubMed: 9566968

Basic Documentation
References

Martin OC, et al. Xgrip109: a gamma tubulin-associated protein with an essential role in gamma tubulin ring complex (gammaTuRC) assembly and centrosome function. J. Cell Biol. 141: 675-687, 1998. PubMed: 9566968