RMC (ATCC® CRL-2573)

Organism: Rattus norvegicus, rat  /  Cell Type: Mesangial Cell  /  Tissue: kidney  / 

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Organism Rattus norvegicus, rat
Tissue kidney
Cell Type Mesangial Cell
Product Format frozen
Culture Properties adherent
Biosafety Level 2 [Cells contain SV40 viral sequences]
Age 3 months
Gender male
Strain Sprague-Dawley
Applications
Mesangial cells are crucial for kidney function and are used extensively in kidney research.

Storage Conditions liquid nitrogen vapor phase
Derivation Rat mesangial cell at passage 8 were immortalized with pSV3-Neo and maintained in the presence of G418.
Clinical Data
male
3 months
Genes Expressed
desmin; vimentin
Cellular Products
desmin; vimentin
Comments
Rat mesangial cell at passage 8 were immortalized with pSV3-Neo and maintained in the presence of G418.
The cells express normal genes of the wild type mesangial cells; they are positive for desmin and vimentin and negative for cytokeratin 8.

Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.4 mg/ml G418, 85%; fetal bovine serum, 15%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:4 to 1:8
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor M Ailenberg, M Silverman
Passage History
Rat mesangial cell at passage 8 were immortalized with pSV3-Neo and maintained in the presence of G418.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation