Hs 835.T (ATCC® CRL-7569)

Organism: Homo sapiens; Mus musculus, human; mouse  /  Cell Type: fibroblast  /  Disease: cancer

Organism Homo sapiens; Mus musculus, human; mouse
Cell Type fibroblast
Product Format frozen
Morphology mixed
Culture Properties adherent
Biosafety Level 1
Disease cancer
Applications
This line was derived from a tumor arising from human renal carcinoma cells injected into a nude mouse.
This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Derivation
This line was derived from a tumor arising from human renal carcinoma cells injected into a nude mouse. Cultures contain fibroblasts (probably murine) and islands of tightly packed tumor cells.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Comments
This line was derived from a tumor arising from human renal carcinoma cells injected into a nude mouse. Cultures contain fibroblasts (probably murine) and islands of tightly packed tumor cells.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Passage History
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.