NCI-H1688 [H1688] (ATCC® CCL-257)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: liver  /  Disease: carcinoma; classic small cell lung cancer

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease carcinoma; classic small cell lung cancer
Age 50 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from a liver metastasis taken from a patient prior to therapy.
Clinical Data
50 years
Caucasian
male
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which   contains trypsin inhibitor. 
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is  dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Subcultivation Ratio: 1:3 to 1:6
  7. Incubate cultures at 37°C.
Medium Renewal: Every 2 to 3 days

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 13
D5S818: 10
D7S820: 12
THO1: 6,8
TPOX: 11
vWA: 17
Name of Depositor AF Gazdar, JD Minna
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation