L.N. 4159 (ATCC® CRL-10998)

Organism: Mus musculus, mouse  / 

Organism Mus musculus, mouse
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Genes Expressed
temperature sensitive T antigen
Cellular Products
temperature sensitive T antigen
Comments
The cells contain the HB101 pLux F70 plasmid.
The cells should be grown at 33C.
Complete Growth Medium Dulbecco's modified Eagle's medium with 200 nM dexamethasone, 96%; fetal bovine serum, 4%
Subculturing
Medium Renewal: Every 2 to 3 days
Remove medium, rinse monolayer with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor Pfizer, Inc.