H4TG (ATCC® CRL-1578)

Organism: Rattus norvegicus, rat  /  Disease: hepatoma

Permits and Restrictions

View Permits

Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatoma
Gender male
Strain AxC
Applications
Phenylalanine hydroxylase is expressed consitutively, and production can be increased by treatment with glucorticoids or cAMP derivatives.
The cells are resistant to 6-thioguanine and sensitive to HAT medium.
They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-).
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of the H4-II-E-C3 rat hepatoma cell line (see ATCC CRL-1600).
Phenylalanine hydroxylase is expressed consitutively, and production can be increased by treatment with glucorticoids or cAMP derivatives.
Clinical Data
male
Comments
This line is a derivative of the H4-II-E-C3 rat hepatoma cell line (see ATCC CRL-1600).
Phenylalanine hydroxylase is expressed consitutively, and production can be increased by treatment with glucorticoids or cAMP derivatives.
The cells are resistant to 6-thioguanine and sensitive to HAT medium.
They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.075 mM 6-thioguanine and 10% fetal bovine serum
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.075 mM 6-thioguanine and 10% fetal bovine serum
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor TR Chen
References

Haggerty DF, et al. Phenylalanine hydroxylase in cultured hepatoxytes. I. Hormonal control of enzyme levels. J. Biol. Chem. 248: 223-232, 1973. PubMed: 4348207

Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Haggerty DF, et al. Phenylalanine hydroxylase in cultured hepatoxytes. I. Hormonal control of enzyme levels. J. Biol. Chem. 248: 223-232, 1973. PubMed: 4348207

Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233